Human TNF-alpha Protein, Research Grade

SKU: orb3158206

Description

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: TNF-alpha
• Reactivity: Human
• Tag: Native
• Molecular Weight: 17.4 kDa
• Expression Region: Val 77 - Leu 233
• Purity: 95%
• Endotoxins: 0.01 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

TNF-alpha

RefSeq:P01375