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Catalog Number | orb420096 |
---|---|
Category | Proteins |
Description | Human TIGIT, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1). |
Target | TIGIT |
Conjugation | Unconjugated |
Tag | C-mFc(mIgG2a) |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAI01290.1 |
UniProt ID | Q495A1 |
MW | 40.1 kDa |
Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Expression Region | Met 22 - Pro 141 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAI01290.1 |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.24 kDa after removal of the signal peptide.The apparent molecular mass of TIGIT-mFc is approximately 40-53 kDa due to glycosylation. | |
Mammalian |
98.00% | |
39.4 kDa (predicted); 45-54 kDa (reducing condition, due to glycosylation) |