Human Thyrotropin Releasing Hormone (TRH) ELISA Kit

SKU: orb776505

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.7 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TRF ELISA Kit, Thyrotropin-Releasing Factor ELISA Kit, Thyroliberin ELISA Kit, Protirelin ELISA Kit, Pro-tirelin ELISA Kit, Prothyroliberin ELISA Kit, TSH-releasing factor ELISA Kit

Quick Database Links

UniProt

P20396