Human Thymosin Beta 4 (Tb4) ELISA Kit

SKU: orb778255

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 4(Tb4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 4(Tb4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 0.88 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TMSB4X ELISA Kit, FX ELISA Kit, PTMB4 ELISA Kit, TB4X ELISA Kit, TMSB4 ELISA Kit, Thymosin,Beta 4,X-Linked ELISA Kit, TB-500 ELISA Kit, Seraspenide ELISA Kit, Hematopoietic system regulatory peptide ELISA Kit

Quick Database Links

UniProt

P62328