Human Thymosin Beta 10 (TMSb10) ELISA Kit

• Catalog Number: orb776853
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 10(TMSb10) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 10(TMSb10).
• Reactivity: Human
• Concentration: 2000 ng/mL
• UniProt ID: P63313
• Tested applications: ELISA
• Application notes: standard: 2000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TMSb10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMSb10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMSb10 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 31.25-2000 ng/mL
• Sensitivity: 13.97 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Alternative names: MIG12 ELISA Kit, TB10 ELISA Kit, PTMB10 ELISA Kit
• Research Area: Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.