Human Thymidine Phosphorylase (TP) ELISA Kit

• Catalog Number: orb775965
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Phosphorylase(TP).
• Reactivity: Human
• Concentration: 100 ng/mL
• UniProt ID: P19971
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.57 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: TYMP ELISA Kit, PDECGF ELISA Kit, ECGF1 ELISA Kit,
• Research Area: Cancer Biology, Protein Biochemistry
• Note: For research use only