Human Thrombospondin 3 (THBS3) ELISA Kit

• Catalog Number: orb779024
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 3(THBS3).
• Reactivity: Human
• Concentration: 100 ng/mL
• UniProt ID: P49746
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THBS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THBS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THBS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THBS3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.59 ng/mL
• Sample Types: Plasma.
• Alternative names: TSP3 ELISA Kit
• Research Area: Hematology
• Note: For research use only
• Expiration Date: Please enquire.