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Human Thrombospondin-1 (TSP-1) ELISA Kit

Human Thrombospondin-1 (TSP-1) ELISA Kit

Catalog Number: orb2668001

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2668001
CategoryAssays and Kits
DescriptionHuman Thrombospondin-1 (TSP-1) ELISA Kit
Range1.56-100 ng/mL
Assay Time3.5h
UniProt IDP07996
Sensitivity0.47 ng/mL
Alternative namesTSP1; Thrombospondin-1p180; THBS1; Thrombospondin
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesplasma
Expiration DatePlease enquire.
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