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Catalog Number | orb2668001 |
---|---|
Category | Assays and Kits |
Description | Human Thrombospondin-1 (TSP-1) ELISA Kit |
Range | 1.56-100 ng/mL |
Assay Time | 3.5h |
UniProt ID | P07996 |
Sensitivity | 0.47 ng/mL |
Alternative names | TSP1; Thrombospondin-1p180; THBS1; Thrombospondin Read more... |
Note | For research use only |
Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | plasma |
Expiration Date | Please enquire. |
Human | |
2.7 ng/ml - 2000 ng/ml | |
2.7 ng/ml |