Human Thrombospondin-1 (TSP-1) ELISA Kit

SKU: orb2668001

Description

Human Thrombospondin-1 (TSP-1) ELISA Kit

Images & Validation

• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.56-100 ng/mL
• Sensitivity: 0.47 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

Quick Database Links

UniProt

P07996