Human TGFBR1 Protein, N-hFc Tag

• Catalog Number: orb689441
• Category: Proteins
• Description: Recombinant human TGFBR1 Protein with N-terminal Human Fc tag
• Target: TGFBR1
• Tag: N-Human Fc Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P36897
• MW: The protein has a predicted molecular mass of 36.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TGFBR1 is approximately 40-53 kDa due to glycosylation.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: AAT5;ACVRLK4;ALK-5;ALK5;ESS1;LDS1;LDS1A;LDS2A;MSSE
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CTLA-4, hFc tagged protein (orb689463) can bind Anti-CTLA4 Neutralizing antibody orb689327 in a linear range of 0.64-80.0 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (orb689463) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Flow cytometry analysis with 1 μg/mL Human CTLA4 Protein, hFc tag (orb689463) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).