Human T-cell surface glycoprotein CD3 zeta Chain (CD247) ELISA Kit

• Catalog Number: orb1173602
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-cell surface glycoprotein CD3 zeta chain(CD247). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T-cell surface glycoprotein CD3 zeta chain(CD247). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-cell surface glycoprotein CD3 zeta chain(CD247), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-cell surface glycoprotein CD3 zeta chain(CD247) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: P20963
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD247. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD247. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD247, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD247 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotei
• Note: For research use only