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Catalog Number | orb1291298 |
---|---|
Category | Assays and Kits |
Description | Human SDMA (Symmetric dimethylarginine) ELISA Kit |
Reactivity | Human |
Concentration | 20 nmol/mL |
Tested applications | ELISA |
Application notes | standard: 20 nmol/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SDMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SDMA in the samples is then determined by comparing the OD of the samples to the standard curve |
Assay Time | 2.5h |
Range | 0.32-20 nmol/mL |
Sensitivity | 0.12 nmol/mL |
Sample Types | EDTA Plasma, Serum |
Alternative names | Symmetric dimethylarginine |
Note | For research use only |