Human Stromal Cell Derived Factor 1 (SDF1) ELISA Kit

SKU: orb775048

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1(SDF1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SDF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CXCL12 ELISA Kit, PBSF ELISA Kit, P-BSF ELISA Kit, SCYB12 ELISA Kit, SDF1-A ELISA Kit, SDF1-B ELISA Kit, SDF1A ELISA Kit, SDF1B ELISA Kit, TLSF-A ELISA Kit, TLSF-B ELISA Kit, TPAR1 ELISA Kit, Pre-B Cell Growth-Stimulating Factor ELISA Kit, Chemokine C-X-C-Motif Ligand 12 ELISA Kit

Quick Database Links

UniProt

P48061