Human Steroid 5 Alpha Reductase 1 (SRD5a1) ELISA Kit

• Catalog Number: orb866996
• Category: Assays and Kits
• Description: Sandwich Human SRD5a1 ELISA Kit
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: P18405
• Sensitivity: 0.06 ng/mL
• Alternative names: 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SRD5a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SRD5a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SRD5a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SRD5a1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.