Human Stem Cell Factor (SCF) ELISA Kit

SKU: orb775045

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor(SCF).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.3 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

KITLG ELISA Kit, MGF ELISA Kit, SF ELISA Kit, KL1 ELISA Kit, Kitl ELISA Kit, FPH2 ELISA Kit, Kit-Ligand ELISA Kit, Steel Factor ELISA Kit, Mast Cell Growth Factor ELISA Kit, Steel factor ELISA Kit, Familial Progressive Hyperpigmentation 2 ELISA Kit

Standard curve for orb775045 ELISA kit.

Quick Database Links

UniProt

P21583