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Human Stanniocalcin 1 (STC1) ELISA Kit

Catalog Number: orb777196

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777196
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stanniocalcin 1(STC1).
Tested applicationsELISA
ReactivityHuman
Range31.25-2000 pg/mL
Assay Time3.5h
Concentration2000 pg/mL
UniProt IDP52823
Sensitivity11.3 pg/mL
Alternative namesStanniocalcin 1 , Stanniocalcin , Stanniocalcin-1
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NoteFor research use only
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STC1 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Human Stanniocalcin 1 (STC1) ELISA Kit

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