Human sRAGE ELISA Kit Discontinued

SKU: orb782253

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to soluble receptor for advanced glycation endproducts,(sRAGE).

Research Area

Cancer Biology, Cardiovascular Research, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 800 pg/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sRAGE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sRAGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sRAGE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sRAGE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 12.5-800 pg/mL
• Sensitivity: 3.93 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

sRAGE

Nazgol Bahreini 1, Maryam Saghafi-Asl 2, Omid Nikpayam 3, Ehsan Safaei 1, Vahideh Sadra 4, Laleh Fakhr 1, Hanieh Beyrampour-Basmenj 5, Parina Asgharian 6, Mohammad Asghari-Jafarabadi Effects of dried okra extract on lipid profile, renal function and some RAGE-related inflammatory genes expression in patients with diabetic nephropathy: A randomized controlled trial Complement Ther Med ., (2024)

PubMed: 38336011

Applications

ELISA

Reactivity

Human