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| Catalog Number | orb777992 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spondin 2(SPON2). |
| Reactivity | Human |
| Concentration | 5000 pg/mL |
| UniProt ID | Q9BUD6 |
| Tested applications | ELISA |
| Application notes | standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPON2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPON2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPON2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPON2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 78.13-5000 pg/mL |
| Sensitivity | 34 pg/mL |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | DIL1 ELISA Kit, Mindin ELISA Kit, M-spondin ELISA Read more... |
| Note | For research use only |
Human SPON2 ELISA Kit [orb405843]
Human
3.12 ng/mL-200 ng/mL
0.78 ng/mL
48 T, 96 T, 10 x 96 T, 5 x 96 T, 24 T- Item 1 of 1
- Item 1 of 1
