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| Catalog Number | orb257550 |
|---|---|
| Category | Proteins |
| Description | Human SOST, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1). |
| Reactivity | Human |
| Tag | N-6×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 22.3 kDa |
| Target | SOST, Sclerostin |
| UniProt ID | Q9BQB4 |
| Protein Sequence | AAI01087.1 |
| Source | Human SOST, His Tag (orb257550) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Gln 24 - Tyr 213 |
| Endotoxins | 1.0 EU per μg |
| NCBI | AAI01087.1 |
| Storage | -20℃ |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | SOST,VBCH Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Wu, Y. et al. Upregulated serum sclerostin level in the T2DM patients with femur fracture inhibits the expression of bone formation/remodeling-associated biomarkers via antagonizing Wnt signaling Eur Rev Med Pharmacol Sci, 21, 470-478 (2017)
SDS-PAGE analysis of Human SOST protein
SOST Protein, Human, Recombinant (His), Biotinylated [orb1954684]
98.00%
22.5 kDa (predicted)
100 μg, 20 μgSOST Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960775]
98.00%
24.2 kDa (predicted). Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgSOST Protein, Human, Recombinant (His & Avi) [orb1960776]
98.00%
24.2 kDa (predicted). Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgSOST Protein, Human, Recombinant (His) [orb1958678]
99.60%
22.5 kDa (predicted); 29.9 kDa (reducing condition, due to glycosylation)
50 μg, 100 μg, 200 μg, 500 μgRecombinant Human SOST/Sclerostin Protein, Avi His Tag [orb1550393]
> 95% by Tris-Bis PAGE
Recombinant Human SOST/Sclerostin Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln24-Tyr213) of Human SOST/Sclerostin fused with a His Tag and Avi tag at the N-terminal.
100 μg
