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Catalog Number | orb1475765 |
---|---|
Category | Proteins |
Description | Recombinant Human Sonic hedgehog protein/SHH(K122R)(C-His) |
Tag | C-His |
Form/Appearance | Supplied as a 0.2 um filtered solution of 500mM PBS, 300mM NaCl, 10%Glycerol, pH 7.4 |
UniProt ID | Q15465 |
Protein Sequence | C25-G198(K122R) |
Source | E. coli |
Expression System | E. coli |
Storage | Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening.Please minimize freeze-thaw cycles. |
Buffer/Preservatives | Supplied as a 0.2 um filtered solution of 500mM PBS, 300mM NaCl, 10%Glycerol, pH 7.4 |
Alternative names | HHG-1; Shh unprocessed N-terminal signaling and C- Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
20.4 kDa | |
Human Sonic Hedgehog, His Tag, premium grade (orb257838) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
Human | |
62.5 pg/mL-4000 pg/mL | |
15.6 pg/mL |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
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