Human SolubleInterleukin 6 Receptor (sIL-6R) ELISA Kit

• Catalog Number: orb776111
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 Receptor(IL6R).
• Reactivity: Human
• Concentration: 120 ng/mL
• Tested applications: ELISA
• Application notes: standard: 120 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.88-120 ng/mL
• Sensitivity: 0.81 ng/mL
• Sample Types: Serum, plasma, tissue homogenates, urine and other biological fluids
• Alternative names: CD126 ELISA Kit, gp80 ELISA Kit, IL6R ELISA Kit, I
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only