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| Catalog Number | orb1146888 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1B(sTNFRSF1B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1B(sTNFRSF1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1B(sTNFRSF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1B(sTNFRSF1B) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Concentration | 10 ng/mL |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTNFRSF1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTNFRSF1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTNFRSF1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTNFRSF1B in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.066 ng/mL |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | CD120B, P75, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR Read more... |
| Note | For research use only |
