Human Soluble CD130 (sCD130) ELISA Kit

SKU: orb1088140

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble CD130(sCD130). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble CD130(sCD130). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble CD130(sCD130), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble CD130(sCD130) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD130. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD130. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD130, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sCD130 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 125-8000 pg/mL
• Sensitivity: 47 pg/mL
• Concentration: 8000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CDw130, CD130, IL6ST, GP130-RAPS, IR6RB, IL6R-Beta, IR6RB, IR6-RB, Interleukin 6 Signal Transducer, Oncostatin M Receptor, Interleukin-6 Receptor Subunit Beta