Human Soluble amyloid precursor Protein α (sAPPα) ELISA Kit

SKU: orb1173621

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to soluble amyloid precursor protein α(sAPPα). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to soluble amyloid precursor protein α(sAPPα). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain soluble amyloid precursor protein α(sAPPα), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of soluble amyloid precursor protein α(sAPPα) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 800 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sAPPα. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sAPPα. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sAPPα, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sAPPα in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF)
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 12.5-800 ng/mL
• Sensitivity: 5.36 ng/mL
• Concentration: 800 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

sAPPα, soluble amyloid precursor protein alpha, sAPP alpha