Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit

SKU: orb777750

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 1(NHE1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NHE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NHE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NHE1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SLC9A1 ELISA Kit, APNH ELISA Kit, Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger ELISA Kit, Antiporter,Na+/H+,Amiloride Sensitive ELISA Kit, Na(+)/H(+) antiporter, amiloride-sensitive ELISA Kit

Quick Database Links

UniProt

P19634