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Human SMPDL3A protein

SKU: orb392499

Description

Recombinant human SMPDL3A protein is produced by our mammalian expression system and the target gene encoding Leu23-Tyr453 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetSMPDL3A
ReactivityHuman
Protein SequenceLPVAPAGGRN PPPAIGQFWH VTDLHLDPTY HITDDHTKVC ASSKGANASN PGPFGDVLCD SPYQLILSAF DFIKNSGQEA SFMIWTGDSP PHVPVPELST DTVINVITNM TTTIQSLFPN LQVFPALGNH DYWPQDQLPV VTSKVYNAVA NLWKPWLDEE AISTLRKGGF YSQKVTTNPN LRIISLNTNL YYGPNIMTLN KTDPANQFEW LESTLNNSQQ NKEKVYIIAH VPVGYLPSSQ NITAMREYYN EKLIDIFQKY SDVIAGQFYG HTHRDSIMVL SDKKGSPVNS LFVAPAVTPV KSVLEKQTNN PGIRLFQYDP RDYKLLDMLQ YYLNLTEANL KGESIWKLEY ILTQTYDIED LQPESLYGLA KQFTILDSKQ FIKYYNYFFV SYDSSVTCDK TCKAFQICAI MNLDNISYAD CLKQLYIKHN YVDHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ASML3A; Acid sphingomyelinase-like phosphodiesterase 3a; ASM-like phosphodiesterase 3a

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human SMPDL3A protein (orb392499)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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