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Human Small Breast Epithelial Mucin (SBEM) ELISA Kit

Catalog Number: orb778834

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778834
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Breast Epithelial Mucin(SBEM).
ReactivityHuman
Concentration20 ng/mL
UniProt IDQ96DR8
Tested applicationsELISA
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SBEM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SBEM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SBEM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SBEM in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.132 ng/mL
Sample TypesSerum, plasma, tissue homogenates, saliva and other biological fluids
Alternative namesMUCL1 ELISA Kit, Mucin-Like Protein 1 ELISA Kit, P
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NoteFor research use only
Human Small Breast Epithelial Mucin (SBEM) ELISA Kit