Human Sialic Acid (SA) ELISA Kit

• Catalog Number: orb782214
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sialic Acid (SA) protein.
• Tested applications: ELISA
• Reactivity: Human
• Range: 7.82-500 µg/mL
• Assay Time: 2.5h
• Concentration: 500 µg/mL
• Sensitivity: 3.83 µg/mL
• Alternative names: SA ELISA Kit
• Note: For research use only
• Application notes: standard: 500 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SA in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Expiration Date: Please enquire.