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| Catalog Number | orb1088241 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to serum indoxyl sulfate(IS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to serum indoxyl sulfate(IS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain serum indoxyl sulfate(IS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of serum indoxyl sulfate(IS) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 31.25-2000 ng/mL |
| Assay Time | 3.5h |
| Concentration | 2000 ng/mL |
| Sensitivity | 12.1 ng/mL |
| Alternative names | IS, indoxyl sulfate, serum indoxyl sulfate Read more... |
| Note | For research use only |
| Application notes | standard: 2000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IS in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |
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