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| Catalog Number | orb1950018 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.78-50 ng/mL |
| Assay Time | 1.5h |
| Concentration | 50 ng/mL |
| UniProt ID | P0DJI8 |
| Sensitivity | 0.15 ng/mL |
| Alternative names | SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amylo Read more... |
| Note | For research use only |
| Application notes | standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma |
| Expiration Date | Please enquire. |
