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| Catalog Number | orb776958 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serglycin(SRGN). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 0.16-10 ng/mL |
| Assay Time | 3.5h |
| Concentration | 10 ng/mL |
| UniProt ID | P10124 |
| Sensitivity | 0.059 ng/mL |
| Alternative names | PRG ELISA Kit, PPG ELISA Kit, PRG1 ELISA Kit, prot Read more... |
| Note | For research use only |
| Application notes | standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SRGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SRGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SRGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SRGN in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |
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Human SRGN ELISA Kit [orb407112]
Human
0.312 ng/mL-20 ng/mL
0.078 ng/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T
