Human Semaphorin 4D (SEMA4D) ELISA Kit

SKU: orb1948254

Description

Human Semaphorin 4D (SEMA4D) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA4D. You can calculate the concentration of Human SEMA4D in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 0.32-20ng/mL
• Sensitivity: 0.19 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD1, M-sema-G, SEMAJ, coll-4

Quick Database Links

Gene Symbol

SEMA4D

UniProt

Q92854