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Catalog Number | orb706846 |
---|---|
Category | Proteins |
Description | Recombinant Human CD162 Protein is produced by mammalian expression system and the target gene encoding Thr44-Gly295 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SELPLG |
Entrez | 6404 |
UniProt ID | Q14242 |
Protein Sequence | TEYEYLDYDF LPETEPPEML RNSTDTTPLT GPGTPESTTV EPAARRSTGL DAGGAVTELT TELANMGNLS TDSAAMEIQT TQPAATEAQT TQPVPTEAQT TPLAATEAQT TRLTATEAQT TPLAATEAQT TPPAATEAQT TQPTGLEAQT TAPAAMEAQT TAPAAMEAQT TPPAAMEAQT TQTTAMEAQT TAPEATEAQT TQPTATEAQT TPLAAMEALS TEPSATEALS MEPTTKRGLF IPFSVSSVTH KGIEGRMDPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5. |
Alternative names | P-selectin glycoprotein ligand 1 protein, PSGL-1 p Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 31.4 kDa after removal of the signal peptide.The apparent molecular mass of CD162-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
Biotin | |
90% | |
32.4 kDa | |
Biotinylated Human PSGL-1, His, (orb750334) is expressed from human 293 cells (HEK293). It contains AA Gln 42 - Cys 320 (Accession # Q14242-1). |
Unconjugated | |
90% | |
30.7 kDa | |
Human PSGL-1, His Tag (orb640168) is expressed from human 293 cells (HEK293). It contains AA Gln 42 - Cys 320 (Accession # Q14242-1). |
> 95% by SDS-PAGE. | |
KMP1811, Recombinant Human SELPLG Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (CD162(Leu18-Cys320)) of human SELPLG (Accession #) fused with a His Tag at the C-terminus. |
≥90% as determined by SDS-PAGE | |
This protein contains the human SELPLG(Met1-Gly295) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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