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Human Selectin, Endothelium (SELE) ELISA Kit

Human Selectin, Endothelium (SELE) ELISA Kit

Catalog Number: orb2668129

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2668129
CategoryAssays and Kits
DescriptionHuman Selectin, Endothelium (SELE) ELISA Kit
Range0.16-10 ng/mL
Assay Time3.5h
UniProt IDP16581
Sensitivity0.068 ng/mL
Alternative namesCD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SELE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SELE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SELE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SELE in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.