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| Catalog Number | orb1807210 |
|---|---|
| Category | Assays and Kits |
| Description | Human Secretory Immunoglobulin A (sIgA) ELISA Kit |
| Target | sIgA |
| Reactivity | Human |
| Application notes | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sIgA. You can calculate the concentration of Human sIgA in the samples by comparing the OD of the samples to the standard curve. |
| Assay Type | Sandwich |
| Assay Time | 3.5H |
| Standard | 20 ng/mL |
| Range | 0.31-20 ng/mL |
| Sensitivity | 0.16 ng/mL |
| Sample Types | serum, plasma, Tissue homogenate and Other biological samples |
| Alternative names | sIgA |
| Note | For research use only |
Human SIgA ELISA Kit [orb406262]
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0.012 μg/mL-50 μg/mL
0.012 μg/mL
48 T, 10 x 96 T, 5 x 96 T, 96 T, 24 T- Item 1 of 1
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Human Secretory Immunoglobulin A (sIgA) ELISA Kit [orb775708]
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0.312-20μg/mL
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Human sIgA (Secretory Immunoglobulin A) ELISA Kit [orb2280374]
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0.313-20ng/ml
0.188ng/ml
96 T, 48 T
