Human Secretory IgA

SKU: orb22122

Description

Chromatographically purified human secretory IgA. Purified human secretory IgA may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.

Images & Validation

• Tested Applications: Control

Key Properties

• Source: Human colostrum. The protein is isolated according to a method described in J. Mestecky and M. Kilian, Immunoglobulin A (IgA), in Methods in Enzymology, Vol 116, pp 37-75, 1985

Storage & Handling

• Storage: This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
• Disclaimer: For research use only

References

Pan, Sung-Ching et al. A randomized, double-blind, controlled clinical trial to evaluate the safety and immunogenicity of an intranasally administered trivalent inactivated influenza vaccine with adjuvant LTh(αK): A phase I study Vaccine, 37, 1994-2003 (2019)

PubMed: 30837170