Human Secretogranin V (SCG5) ELISA Kit

SKU: orb777568

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin V(SCG5).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCG5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCG5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCG5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCG5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.6 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SCGV ELISA Kit, P7B2 ELISA Kit, SGNE1 ELISA Kit, SgV ELISA Kit, 7B2 Protein ELISA Kit, Neuroendocrine protein 7B2 ELISA Kit, Prohormone Convertase Chaperone ELISA Kit, Pituitary polypeptide ELISA Kit, Secretory granule endocrine protein I ELISA Kit