Human Runt Related Transcription Factor 2 (RUNX2) ELISA Kit

SKU: orb775595

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt Related Transcription Factor 2(RUNX2).

Research Area

Metabolism Research, Musculoskeletal & Connective Tissue Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RUNX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RUNX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RUNX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RUNX2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

AML3 ELISA Kit, CBFA1 ELISA Kit, CCD1 ELISA Kit, OSF2 ELISA Kit, PEBP2A ELISA Kit, Acute myeloid leukemia 3 ELISA Kit, Core-binding factor alpha-1 ELISA Kit, Osteoblast-specific transcription factor 2 ELISA Kit, Polyomavirus enhancer-binding protein 2 A ELISA Kit

Quick Database Links

UniProt

Q13950