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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−
Key Properties
−| Expression System | Human cells |
|---|---|
| Biological Activity | Cell Based Assay: Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a V3 (Cat# orb3002512). (Regularly tested) |
| Tag | C-Flag |
| Expression Region | Gln22-Gly205 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 22.3 KDa. Observed: 22-28 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−R-spondin-2; RSPO2; Roof plate-specific spondin-2; hRspo2; PRO34209
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide