Recombinant Human RSPO2 Protein

SKU: orb3002513

Description

Recombinant Human R-spondin-2 is produced by our Mammalian expression system and the target gene encoding Gln22-Gly205 is expressed with a Flag tag at the C-terminus.

Research Area

Stem Cell & Developmental Biology

Key Properties

• Expression System: Human cells
• Biological Activity: Cell Based Assay: Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a V3 (Cat# orb3002512). (Regularly tested)
• Tag: C-Flag
• Expression Region: Gln22-Gly205
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 22.3 KDa. Observed: 22-28 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

R-spondin-2; RSPO2; Roof plate-specific spondin-2; hRspo2; PRO34209

Quick Database Links

UniProt

Q6UXX9