Human RHOB Protein, hFc Tag

• Catalog Number: orb1743314
• Category: Proteins
• Description: Recombinant Human RHOB Protein with C-terminal human Fc tag
• Reactivity: Human
• Tag: C-Human Fc Tag
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH 8.0.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 47.4 kDa after removal of the signal peptide. The apparent molecular mass of RHOB-hFc is approximately 35-55 kDa due to glycosylation.
• Target: RHOB
• UniProt ID: P62745
• Source: E.coli
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: ARH6;ARHB;MST081;MSTP081;RHOH6
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Greater than 95% as determined by reducing SDS-PAGE.

Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08 ng/ml.

Loaded Human IL-7RA-His on HIS1K Biosensor, can bind Human IL-7 with an affinity constant of 15.04 nM as determined in BLI assay.

Loaded Mouse IL-7RA-Fc on Protein A Biosensor, can bind Human IL-7 with an affinity constant of 29.2 nM as determined in BLI assay.