Human Rho GDP Dissociation Inhibitor Beta (ARHGDIb) ELISA Kit

SKU: orb778411

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Beta(ARHGDIb).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARHGDIb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARHGDIb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARHGDIb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARHGDIb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.233 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

D4 ELISA Kit, GDIA2 ELISA Kit, GDID4 ELISA Kit, LYGDI ELISA Kit, Ly-GDI ELISA Kit, RAP1GN1 ELISA Kit, RhoGDI2 ELISA Kit

Quick Database Links

UniProt

P52566