Human Resistin (RETN) ELISA Kit

• Catalog Number: orb775090
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin(RETN).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q9HD89
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RETN in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit,
• Note: For research use only

References

Amir Ghaffari Jolfayi 1, Ahmad Tashakori Beheshti 1, Seyyed Mohammadjavad Hosseini 1, Amir Akbar Fakhrabadi 1, Bahram Mohebbi 1, Mahshid Malakootian 2, Majid Maleki 2, Hamidreza Pouraliakbar 1, Saeid Hosseini 3, Maedeh Arabian Epicardial adipose tissue features as a biomarker and therapeutic target in coronary artery disease Sci Rep ., (2025)

PubMed: 40295726

Applications

ELISA

Reactivity

Human