Human Resistin Like Beta (RETNLb) ELISA Kit

SKU: orb780438

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta(RETNLb).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RETNLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RETNLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RETNLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RETNLb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 23.3 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

XCP2 ELISA Kit, FIZZ2 ELISA Kit, HXCP2 ELISA Kit, CCRG ELISA Kit, RETNL2 ELISA Kit, RELMb ELISA Kit, Foundin In Flammatory Zone 2 ELISA Kit, Colon and small intestine-specific cysteine-rich protein ELISA Kit, Colon carcinoma-related gene protein ELISA Kit

Quick Database Links

UniProt

Q9BQ08