Human Replication Initiator 1 (REPIN1) ELISA Kit

SKU: orb777640

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1(REPIN1).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REPIN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REPIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REPIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REPIN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.132 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

AP4 ELISA Kit, RIP60 ELISA Kit, ZNF464 ELISA Kit, Zfp464 ELISA Kit, Replication Initiation Region Protein(60kD) ELISA Kit, Zinc Finger Protein 464 ELISA Kit, 60 kDa origin-specific DNA-binding protein ELISA Kit, ATT-binding protein ELISA Kit

Quick Database Links

UniProt

Q9BWE0