Human Pyruvate Kinase, Liver and RBC (PK) ELISA Kit

• Catalog Number: orb775786
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Liver And RBC(PK).
• Tested applications: ELISA
• Reactivity: Human
• Range: 39.07-2500 pg/mL
• Assay Time: 3.5h
• Concentration: 2500 pg/mL
• UniProt ID: P30613
• Sensitivity: 17 pg/mL
• Alternative names: PKLR ELISA Kit, PyK ELISA Kit, PK1 ELISA Kit, PKL
• Note: For research use only
• Application notes: standard: 2500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PK in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.