Human Pulmonary surfactant-Associated Protein D (SP-D) ELISA Kit

• Catalog Number: orb782355
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-D(Pulmonary surfactant-associated protein D).
• Reactivity: Human
• Concentration: 80 ng/mL
• UniProt ID: P35247
• Tested applications: ELISA
• Application notes: standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 1.25-80 ng/mL
• Sensitivity: 0.53 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
• Alternative names: SFTPD ELISA Kit, SPD ELISA Kit, SFTP 4 ELISA Kit,
• Note: For research use only