Human PSMa7(Proteasome Subunit Alpha Type 7) Microsample ELISA Kit

SKU: orb2998286

Description

Human PSMa7(Proteasome Subunit Alpha Type 7) Microsample ELISA Kit

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMa7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMa7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMa7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMa7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

C6; HSPC; RC6-1; XAPC7

Quick Database Links

UniProt

O14818