Human Prothrombin (PT) ELISA Kit

SKU: orb1948641

Description

Human Prothrombin (PT) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PT. You can calculate the concentration of Human PT in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: plasma, Becomes thrombin in serum
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 9.38-600ng/mL
• Sensitivity: 5.63 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Prothrombin,coagulation factor II, F2, PT, RPRGL2, THPH1

Quick Database Links

Gene Symbol

PT

UniProt

P00734