Human Proteolipid Protein 1, Myelin (PLP1) ELISA Kit

SKU: orb778628

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteolipid Protein 1, Myelin(PLP1).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MMPL ELISA Kit, PLP/DM20 ELISA Kit, PMD ELISA Kit, SPG2 ELISA Kit, Pelizaeus-Merzbacher Disease ELISA Kit, Spastic Paraplegia 2,Uncomplicated ELISA Kit, Myelin proteolipid protein ELISA Kit, Lipophilin ELISA Kit

Quick Database Links

UniProt

P60201