Human Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit

SKU: orb2667957

Description

Human Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit

Images & Validation

• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.56-100 ng/mL
• Sensitivity: 0.46 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

P58; ERp61; ERp57; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein,58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57

Quick Database Links

UniProt

P30101