Human Prostate Specific membrane Antigen (PSMA) EasyStep ELISA Kit

• Catalog Number: orb1950024
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMA, and the Human PSMA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSMA. After TMB substrate solution is added, only those wells that contain Human PSMA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMA in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 40 ng/mL
• UniProt ID: Q04609
• Application notes: standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMA, and the Human PSMA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSMA. After TMB substrate solution is added, only those wells that contain Human PSMA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMA in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.165 ng/mL
• Sample Types: Serum, plasma
• Alternative names: Cell growth inhibiting protein 27; Cell growth-inh
• Research Area: Cancer Biology, Metabolism Research, Signal Transd
• Note: For research use only